Hirotsu Bio Science Inc. | HIROTSUバイオサイエンス

2024

A non-invasive screening method using Caenorhabditis elegans for early detection of multiple cancer types: A prospective clinical study

Hideyuki Hatakeyama, Masayo Morishita, Aya Hasan Alshammari, Umbhorn Ungkulpasvich, Junichi Yamaguchi, Takaaki Hirotsu, Eric di Luccio

Biochemistry and Biophysics Reports,September 2024, 39, 101778 (peer-reviewed)

Structural insights into the C-terminus of the histone-lysine N-methyltransferase NSD3 by small-angle X-ray scattering

Benny Dannilo Belviso, Yunpeng Shen, Benedetta Carrozzini, Masayo Morishita, Eric di Luccio, Rocco Caliandro

Frontiers in Molecular Biosciences, 07 March 2024, DOI: 10.3389/fmolb.2024.1191246 (peer-reviewed)

2023

Clinical Possibility of Caenorhabditis elegans as a Novel Evaluation Tool for Esophageal Cancer Patients Receiving Chemotheraoy:A Prospective Study

Yuta Sato, Manabu Futamura, Yoshihiro Tanaka, Hiroshi Tsuchiya, Masahiro Fukada, Toshiya Higashi, Itaru Yasufuku, Ryuichi Asai, Jesse Yu Tajima, Shigeru Kiyama, Hideyuki Hatakeyama, Masayo Morishita, Takaaki Hirotsu, Eric di Luccio, Takuma Ishihara, Nobuhisa Matsuhashi, Kazuhiro Yoshida

Cancers, 2023, 15(15), 3870（peer-reviewed）

2022

Pediatric cancer screening using nematode C. elegans

Toko Shinkai, Toshimi Sugimoto, Kouji Masumoto, Takaaki Hirotsu

Pediatric Surgery, 2022, Vol.54, No.10,1032-1035

A new detection method for canine and feline cancer using the olfactory system of nematodes

Toshimi Sugimoto, Yozo Okuda, Ayaka Shima, Natsuko Sugiura, Nobuaki Kondo, Genki Ishihara, Takaaki Hirotsu, Eric di Luccio

Biochemistry and Biophysics Reports, Volume 32, December 2022.（peer-reviewed）

2021

Scent test using Caenorhabditis elegans to screen for early-stage pancreatic cancer

Ayumu Asai, Masamitsu Konno, Miyuki Ozaki, Koichi Kawamoto, Ryota Chijimatsu, Nobuaki Kondo, Takaaki Hirotsu and Hideshi Ishii

Oncotarget. 2021; 12:1687-1696.（peer-reviewed）

Caenorhabditis elegans as a Diagnostic Aid for Pancreatic Cancer

Masanori Kobayashi, Akashi Fujita, Tomoya Ogawa, Yuki Tanisaka, Masafumi Mizuide, Nobuaki Kondo, Yuki Imaizumi, Takaaki Hirotsu, Shomei Ryozawa

Pancreas. 2021 May-Jun 01;50(5):673-678.（peer-reviewed）

A study on the detectability of digestive system cancers by N-NOSE(Nematode-NOSE)

Ko Nishikawa, Akihiko Tsuchiya, Yoriyuki Takamori, Youji Harada, Toshiya Horibe, Takaaki Hirotsu

Journal of Gastrointestinal Cancer Screening, 2021,59(3),237-245（peer-reviewed）

Accuracy evaluation of the C. elegans cancer test (N-NOSE) using a new combined method

Satoshi Inaba, Naoki Shimozono, Hidehiko Yabuki, Motoki Enomoto, Masayo Morishita, Takaaki Hirotsu, Eric diLuccio*

Cancer Treatment and Research Comunication, 2021, 27:100370（peer-reviewed）

2020

COVID-19 Drug Discovery Using Intensive Approaches

Ayumu Asai, Masamitsu Konno, Miyuki Ozaki, Chihiro Otsuka, Andrea Vecchione, Takahiro Arai, Toru Kitagawa, Ken Ofusa, Masami Yabumoto, Takaaki Hirotsu, Masateru Taniguchi, Hidetoshi Eguchi, Yuichiro Doki and Hideshi Ishii*

Int. J. Mol. Sci. 2020, 21(8), 2839（peer-reviewed）

Efficiency of Gastrointestinal Cancer Detection by Nematode-NOSE (N-NOSE)

Hirotake Kusumoto, Kotaro Tashiro, Syunji Shimaoka, Koichiro Tsukasa, Yukiko Baba, Saori Furukawa, Junichiro Furukawa, Toyokuni Suenaga, Masaki Kitazono, Sadao Tanaka, Toru Niihara, Takaaki Hirotsu, *Takayuki Uozumi

In vivo, vol. 34 no. 1 73-80（peer-reviewed）

2019

Behavioural Response Alteration in Caenorhabditis elegans to Urine After Surgical Removal of Cancer: Nematode-NOSE (N-NOSE) for Postoperative Evaluation

Hirotake Kusumoto, Kotaro Tashiro, Syunji Shimaoka, Koichiro Tsukasa, Yukiko Baba, Saori Furukawa, Junichiro Furukawa, Toyokuni Suenaga, Masaki Kitazono, Sadao Tanaka, Toru Niihara, Takaaki Hirotsu, *Takayuki Uozumi

Biomarkers in Cancer（peer-reviewed）

Application of C. elegans cancer screening test for the detection of pancreatic tumor in genetically engineered mice

Yuji Ueda, Koichi Kawamoto, Masamitsu Konno, Kozo Noguchi, Satoru Kaifuchi, Taroh Satoh, Hidetoshi Eguchi, Yuichiro Doki, Takaaki Hirotsu, Masaki Mori and Hideshi Ishii

Oncotarget 2019 Vol,10 No.52 pp5412-5418（peer-reviewed）

2015

A Highly Accurate Inclusive Cancer Screening Test Using Caenorhabditis elegans Scent Detection

*Hirotsu T., Sonoda H., Uozumi T., Shinden Y., Mimori K., Maehara Y., Ueda N., Hamakawa M.

PLOS ONE, 10(3): e0118699（peer-reviewed）

Voltage-dependent anion channel (VDAC-1) is required for olfactory sensing in C. elegans

Uozumi T., Hamakawa M., Deno Y., Nakajo M., *Hirotsu T.

Genes to Cells, 20, 802-816（peer-reviewed）

A role for Ras in inhibiting circular foraging behavior as revealed by a new method for time and cell-specific RNAi.

Hamakawa M., Uozumi T., Ueda N., Iino Y, *Hirotsu T.

BMC Biology, 13:6（peer-reviewed）

Cancer screening test using C. elegans scent detection.

*Hirotsu T.

Aroma Research, 16, 134-136（invitation）

2014

Screening of Odor-Receptor Pairs in Caenorhabditis elegans Reveals Different Receptors for High and Low Odor Concentrations.

Taniguchi G., Uozumi T., Kiriyama K., Kamizaki T., *Hirotsu T.

Science Signaling, 7 (323), ra39（peer-reviewed）

Screening of odor-receptor pairs reveals different receptors function depending on odor concentrations.

*Hirotsu T.

Aroma Research, 15, 242-243（invitation）

2013

In vivo imaging of Ras protein’s activity in olfactory neurons.

Uozumi T., Yamada R., Suzuki A., Taniguchi G., Yoshida K., Iino Y., Ishihara T., *Hirotsu T.

Protocol Exchange, 2013, 011（invitation）

2012

Temporally-regulated quick activation and inactivation of Ras is important for olfactory behaviour.

†Uozumi T., †*Hirotsu T., Yoshida K., Yamada R., Suzuki A., Taniguchi G., Iino Y., and Ishihara T.

(Nature) Scientific Reports, 2, 500（peer-reviewed）

Odour concentration-dependent olfactory preference change in C. elegans.

Yoshida K., *Hirotsu T., Tagawa T., Oda S., Wakabayashi T., Iino Y., and Ishihara T.

Nature Communications, 3, 739（peer-reviewed）

Live imaging of signaling protein’s activity responding to odorant stimuli.

Uozumi T., *Hirotsu T

Aroma Research, 13, 244（invitation）

Neural mechanisms of olfactory preference change depending on the odor concentration.

Yoshida K., *Hirotsu T.

Aroma Research, 13, 137（invitation）

Scent aroma derived from woods: the consideration based on the physiological and psychological effects.

Shimizu K., Hirotsu T. (8th author) et al.

Aroma Research, 13, 202-208（invitation）

Comprehensive analysis of olfactory receptors in C. elegans

*Hirotsu T., Taniguchi G., Kiriyama K., Kamizaki T., Sato N. and Ishihara T.

Genetics in 21th Century, VIII, 12（invitation）

2011

Behavioral choice between conflicting alternatives is regulated by a receptor guanylyl cyclase GCY-28 and a receptor tyrosine kinase SCD-2 in AIA interneurons of C. elegans.

Shinkai Y., Yamamoto Y., Fujiwara M., Tabata T., Murayama T., Hirotsu T., Ikeda D., Tsunozaki M., Iino Y., Bargmann C., Katsura I., and Ishihara T.

Journal of Neuroscience, 31, 3007-3015

2010

Olfactory plasticity is regulated by pheromonal signaling in Caenorhabditis elegans.

Yamada K., Hirotsu T., Matsuki M., Butcher A., Tomioka M., Ishihara T., Clardy J., Kunitomo H. and Iino Y.

Science, 329, 1647-1650（peer-reviewed）

2009

A trophic role for Wnt-Ror kinase signaling during developmental pruning in Caenorhabditis elegans.

Hayashi Y., Hirotsu T., Iwata R., Kage-Nakadai E., Kunitomo H., Ishihara T., Iino Y. and Kubo T.

Nature Neuroscience, 12, 981-987（peer-reviewed）

Behavioural assay for olfactory plasticity in C. elegans.

Hirotsu T., Hayashi Y., Iwata R., Kunitomo H., Kage-Nakadai E., Kubo T., Ishihara T. and Iino Y.

Nature Protocols, 2009, 139（invitation）

GPC-1, a G Protein {gamma} Subunit, Regulates Olfactory Adaptation in Caenorhabditis elegans.

Yamada K., Hirotsu T., Matsuki M., Kunitomo H. and Iino Y.

Genetics, 181, 1347-1357（peer-reviewed）

2008

Analysis of neural mechanisms of behavioral changes depending on odor concentrations.

Yoshida K., *Hirotsu T., Ishihara T. and Iino Y.

Genetics in 21th Century, V, 5（invitation）

2005

Neural circuit-dependent odor adaptation in C. elegans is regulated by the Ras-MAPK pathway.

Hirotsu T. and Iino Y.

Genes to Cells, 10, 517-530（peer-reviewed）

MBR-1, a novel helix-turn-helix transcription factor, is required for pruning of excessive neurites in Caenorhabditis elegans.

Kage E., Hayashi Y., Takeuchi H., Hirotsu T., Kunitomo H., Inoue T., Arai H., Iino Y. and Kubo T.

Current Biology, 15, 1554-1559（peer-reviewed）

2000

The Ras-MAPK pathway is important for olfaction in Caenorhabditis elegans.

Hirotsu T., Saeki S., Yamamoto M. and Iino Y.

Nature, 404, 289-93（peer-reviewed）

1999

Novel functions of the Ras-MAPK signal transduction pathway in neurons of C. elegans.

Hirotsu T., Saeki S. and Iino Y.

Worm Breeder's Gazette,15, 41

Achievements

ACADEMIC PAPERS

A non-invasive screening method using Caenorhabditis elegans for early detection of multiple cancer types: A prospective clinical study

Structural insights into the C-terminus of the histone-lysine N-methyltransferase NSD3 by small-angle X-ray scattering

Clinical Possibility of Caenorhabditis elegans as a Novel Evaluation Tool for Esophageal Cancer Patients Receiving Chemotheraoy:A Prospective Study

Pediatric cancer screening using nematode C. elegans

A new detection method for canine and feline cancer using the olfactory system of nematodes

Scent test using Caenorhabditis elegans to screen for early-stage pancreatic cancer

Caenorhabditis elegans as a Diagnostic Aid for Pancreatic Cancer

A study on the detectability of digestive system cancers by N-NOSE(Nematode-NOSE)

Accuracy evaluation of the C. elegans cancer test (N-NOSE) using a new combined method

COVID-19 Drug Discovery Using Intensive Approaches

Efficiency of Gastrointestinal Cancer Detection by Nematode-NOSE (N-NOSE)

Behavioural Response Alteration in Caenorhabditis elegans to Urine After Surgical Removal of Cancer: Nematode-NOSE (N-NOSE) for Postoperative Evaluation

Application of C. elegans cancer screening test for the detection of pancreatic tumor in genetically engineered mice

A Highly Accurate Inclusive Cancer Screening Test Using Caenorhabditis elegans Scent Detection

Voltage-dependent anion channel (VDAC-1) is required for olfactory sensing in C. elegans

A role for Ras in inhibiting circular foraging behavior as revealed by a new method for time and cell-specific RNAi.

Cancer screening test using C. elegans scent detection.

Screening of Odor-Receptor Pairs in Caenorhabditis elegans Reveals Different Receptors for High and Low Odor Concentrations.

Screening of odor-receptor pairs reveals different receptors function depending on odor concentrations.

In vivo imaging of Ras protein’s activity in olfactory neurons.

Temporally-regulated quick activation and inactivation of Ras is important for olfactory behaviour.

Odour concentration-dependent olfactory preference change in C. elegans.

Live imaging of signaling protein’s activity responding to odorant stimuli.

Neural mechanisms of olfactory preference change depending on the odor concentration.

Scent aroma derived from woods: the consideration based on the physiological and psychological effects.

Comprehensive analysis of olfactory receptors in C. elegans

Behavioral choice between conflicting alternatives is regulated by a receptor guanylyl cyclase GCY-28 and a receptor tyrosine kinase SCD-2 in AIA interneurons of C. elegans.

Olfactory plasticity is regulated by pheromonal signaling in Caenorhabditis elegans.

A trophic role for Wnt-Ror kinase signaling during developmental pruning in Caenorhabditis elegans.

Behavioural assay for olfactory plasticity in C. elegans.

GPC-1, a G Protein {gamma} Subunit, Regulates Olfactory Adaptation in Caenorhabditis elegans.

Analysis of neural mechanisms of behavioral changes depending on odor concentrations.

Neural circuit-dependent odor adaptation in C. elegans is regulated by the Ras-MAPK pathway.

MBR-1, a novel helix-turn-helix transcription factor, is required for pruning of excessive neurites in Caenorhabditis elegans.

The Ras-MAPK pathway is important for olfaction in Caenorhabditis elegans.

Novel functions of the Ras-MAPK signal transduction pathway in neurons of C. elegans.

Proceedings papers

The neural circuit mechanisms in the olfactory preference change after odor experience

The Ras-MAPK pathway is important for foraging behavior in C. elegans

Systematic identification of the relationship between olfactory receptors and odorants in C. elegans

Identification of neural circuits of central olfactory adaptation in C. elegans

Best Paper award3D-03 Systematic identification of specific receptors for odorants in C. elegans

In vivo imaging of Ras activity in olfactory neurons suggests its transient activation is important for olfactory behaviour

Coordinated change of acting sensory neurons is important for olfactory preference change depending upon odor concentration

Live imaging of Ras activity in olfactory neurons in C. elegans.

Analysis of putative olfactory receptor genes in C. elegans.

Best Paper awardAnalysis of a neural circuit for the aversive response to high concentration of an attractive odorant in C. elegans.

The neprilysin gene nep-2 is involved in olfactory adaptation in C. elegans.

G protein signaling in olfactory systems of C. elegans.

The neprilysin gene nep-2 is involved in olfactory adaptation in C. elegans.

Glutamate receptors are regulated by the Ras-MAPK pathway in neural circuit-dependent odor adaptation in C. elegans.

Analysis of the significance and mechanism of neurite elimination using C. elegans as a model.

A novel C. elegans transcription factor MBR-1 is involved in olfactory plasticity and axon pruning.

The Ras-MAPK pathway controls neural circuit-dependent odor adaptation by regulating localization of glutamate receptors in C. elegans.

Analysis of the function of MBR-1, a C. elegans transcription factor incolved in olfactory plasticity , in axon pruning.